book: Alcohol Dependence at the Cellular Level: Effects of Ethanol on Calcium Homeostasis of IM-9...

book: Alcohol Dependence at the Cellular Level: Effects of Ethanol on Calcium Homeostasis of IM-9 Human Lymphoblast ... | Jozsef Nagy

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Alcohol Dependence...

	Alcohol Dependence at the Cellular Level: Effects of Ethanol on Calcium Homeostasis of IM-9 Human Lymphoblast ... Jozsef Nagy Alcohol Research Documentation, Inc., 2000
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This digital document is an article from Journal of Studies on Alcohol, published by Alcohol Research Documentation, Inc. on March 1, 2000. The length of the article is 3510 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.

From the author: Objective: Increased intracellular calcium level ([[[Ca.sup.2+]].sub.i]) was previously observed in peripheral blood mononuclear cells of alcoholic patients under withdrawal. To determine whether this change in [[[Ca.sup.2+]].sub.i] is due to direct effect of ethanol on blood cells and shows adaptation of these cells to alcohol, we examined the effect of alcohol on IM-9 human lymphoblast cultures. Method: [[[Ca.sup.2+]].sub.i] of IM-9 lymphoblasts, after acute and chronic alcohol treatment as well as alcohol withdrawal, was measured fluorometrically using Quin2 as a [Ca.sup.2+]-indicator. Results: Acute ethanol treatment induced a rapid, transient increase in intracellular calcium concentration in a dose-dependent manner. During chronic ethanol (50mM) administration, after the first 24 hours, basal [[[Ca.sup.2+]].sub.i] was significantly lower (44 [+ or -] 8% of control, p [is less than] .025), whereas from the fifth day [[[Ca.sup.2+]].sub.i] was significantly higher (415 [+ or -] 59% of control on the sixth day, p [is less than] .01) compared with untreated cells. After a 3-day ethanol (50mM or 100mM) treatment, elimination of alcohol from the medium caused elevation in [[[Ca.sup.2+]].sub.i]. After 24-hour ethanol withdrawal, the addition of alcohol decreased the elevated [[[Ca.sup.2+]].sub.i] of alcohol-adapted cells to the resting level of control cells. Similar results were observed when verapamil, diazepam or haloperidol were applied. Moreover, when ethanol or these agents were administered at the beginning of alcohol withdrawal, they could prevent the increase in [[[Ca.sup.2+]].sub.i]. Conclusions: These results suggest that alcohol dependence can develop at the cellular level, and that changes in calcium homeostasis, likely due to direct effects of ethanol on ion channels, may play a central role in processes leading to adaptation of cells to alcohol. (J. Stud. Alcohol 61: 225-231, 2000)

Citation Details
Title: Alcohol Dependence at the Cellular Level: Effects of Ethanol on Calcium Homeostasis of IM-9 Human Lymphoblast Cells(*).(Statistical Data Included)
Author: Jozsef Nagy
Publication: Journal of Studies on Alcohol (Refereed)
Date: March 1, 2000
Publisher: Alcohol Research Documentation, Inc.
Volume: 61 Issue: 2 Page: 225

Article Type: Statistical Data Included

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